In general, the parasite eggs or larvae included in ancient samples were studied in two different ways: microscopic and molecular methods. Of course, simple decision like the presence of A. lumbricoides or T. trichiura could be also done in my lab. However, we need a collaborator-paleoparasitologist with higher technical skills for examining the samples in much details (e.g. finding rare parasite eggs within the samples). We already introduce Dr. Seo M., our collaborator for paleoparasitological study, in different page.
When the samples were collected in archaeological fields, they were treated for microscopic examination as follows:
1. Re-hydrated in 0.5% trisodium phosphate solution that had been periodically shaken over the course of one week.
2. The re-hydrated samples, after filtration through multiply layered gauze, were precipitated for one additional day.
3. After the upper turbid layer was discarded, the…