New Paper: ASCARIS ANCIENT DNA SEQUENCES FROM JOSEON MUMMY FECES OF KOREA
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We analyzed Ascaris ancient DNA (aDNA) of cytochrome b (cyt b), cytochrome c oxidase subunit 1 (COX1), NADH dehydrogenase subunit 1 (NAD1) and internal transcribed spacer 1 (ITS1) genes extracted from the feces or precipitates of 15th to 18th century Korean mummies. After multiple Ascaris genes in ancient samples were successfully amplified by PCR, consensus sequences could be determined by the alignment of the sequences of cloned PCR products. The obtained sequences of each gene were highly similar to those of Ascaris spp. reported thus far, but were genetically distinct from Baylisascaris, Parascaris, and Toxascaris spp. The current report establishes that the genetic characteristics of the Ascaris spp. infecting pre-modern Korean societies were not uniform but rather were diverse to some degree.